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BOTANICA SERBICA

The influence of gypsiferous substrata on bryophyte growth: are there obligatory gypsophilous.
BOGDANOVIĆ M, SABOVLJEVIĆ M, SABOVLJEVIĆ A & GRUBIŠIĆ D.
Pages 75-82

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ABSTRACT: In this paper the effects of gypsum (CaSO₄ x 2H₂O) in the growth medium were tested on two non-gypsophilous selected bryophyte species: Bryum argenteum Hedw. and Atrichum undulatum (Hedw.) P. Beauv. With aim to test if some bryophytes are exclusive for gypsum enriched substrates, the difference in gypsum effect on moss development were examined. In vitro cultures of two selected species were initiated from nearly mature spores within unopened capsules. Half strenght Murashige and Skoog media with added gypsum in concentrations of 50, 350 and 500mM were used to test bryophyte develepment. Plants were grown on media with gypsum for 3 days or 3 weeks to compare short and long term effect of salt exposure. Tested bryophytes, non-adapted to gypsum and exposed to various gypsum concentrations in MS medium, did not show to have a problem in surviving the new environment conditions. Moss species selected in this study and exposed to gypsum enriched MS medium showed variation in morphological parameters to some extent (index of multiplication, secondary protonemal production and survival rate) and chlorophyll content and slightly altered chlorophyll a/b ratio. In general, both tested species could survive gypsum enriched medium: secondary protonema was developed and new shoots were formed in both species, but slight differences were recorded between short and long term exposure to gypsum. Acording to data obtained, gypsum is not the stressor (at least not alone) which can separate some interesting bryophytes into the ecological group of gypsophytes.

KEY WORDS: bryophytes, mosses, gypsum, gypsophytes, Atrichum undulatum, Bryum argenteum

Effects of day length on photosynthetic pigments and antioxidative metabolism of in vitro cultured Atrichum undulatum (Hedw.) P. Beauv. (Bryophyta)
CVETIĆ T, SABOVLJEVIĆ A, BOGDANOVIĆ PRISTOV J & SABOVLJEVIĆ M.
Pages 83-88

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ABSTRACT: Photoperiod is known to regulate many essential processes in plants, but physiological eff ects of photoperiod in vegetative stage of plant life have seldom been studied. Th is paper deals with eff ects of day length on Catherine’s moss grown in aseptic culture. Photosynthetic pigments did not show signifi cant variations as a consequence of growth in diff erent photoperiods. Protein content and malate dehydrogenase activity were higher in long day (16h light/8h dark) than in short day (8h light/16h dark) grown plants. Total phenolic compounds contents, as well as total antioxidative capacity were shown to be higher in plants grown in long day conditions. Peroxidase activity was also higher in long day than in short day grown plants. Regulation of components of antioxidative metabolism in a moss species grown in diff erent photoperiods are discussed in relation to same parameters in higher plants.

KEY WORDS: moss, Atrichum undulatum, photoperiod, vegetative phase, antioxidative metabolism

Axenically culturing the bryophytes: a case study of the moss Dicranum scoparium Hedw. (Dicranaceae, Bryophyta)
VUJIČIĆ M, SABOVLJEVIĆ A & SABOVLJEVIĆ M.
Pages 137-140

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ABSTRACT: The aim of this study was to establish axenic culture of the moss Dicranum scoparium a counterpart of rare and widly endangered D. viride. The media contents, as well as light and temperature were varied to find the optimal conditions for spore germination, protonema growing, bud formation and gametophyte development. The best contitions for micropropagation or axenically bryo-farming is to grow D. scoparium on the MS medium enriched with sucrose (1.5%), at 18-20°C independent of light length condition.

KEY WORDS: moss, Dicranum scoparium, in vitro, axenical culture


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