Home
Editorial Board
For the Authors
Contact
Archives
Bibliography of Previous Issues
Institute of Botany and Botanical Garden "Jevremovac"
Faculty of Biology



    BOTANICA SERBICA
    Danova, K.


In vitro culture of Balkan endemic and rare Pulsatilla species for conservational purposes and secondary metabolites production
DANOVA K, BERTOLI A, PISTELLI L, DIMITROV D & PISTELLI L.
Pages 157-162

Full reference | Abstract | Full text PDF
    ABSTRACT: Shoot cultures of Pulsatilla montana ssp. balcana, P. halleri ssp. rhodopaea and P. slaviankae (Zimm.) Jordanov & Kozuharov were initiated from surface-sterilized seeds of the three species, collected at their natural habitats. Shoot cultures of the three species in Murashige and Skoog’s culture medium (MS) exhibited a chlorophyll a/b (chl a/b) ratio ranging from 1, 86 to 3, 01. Moreover by using different concentrations of benzyladenine and 3-indole butyric acid the chl a/b ratio differed from species to species, showing a different adaptability of species to different media. The shoot cultures of the three Pulsatilla species were also analyzed as an in vitro source of polyphenolic antioxidant substances so they were screened for total phenolics, flavonoids and anthocyanidins content in three media variants, demonstrating the highest content for these substances in P. montana ssp. balcana and P. halleri ssp. rhodopaea in the growth regulator-free and 6-benzyladenine – supplemented medium, while in medium supplemented with auxin and cytokinin all three species produced commensurable levels of the studied metabolites.

    KEY WORDS: Pulsatilla montana ssp. balcana, P. halleri ssp. rhodopaea, P. slaviankae (Zimm.) Jordanov & Kozuharov, shoot cultures, antioxidant phenolics, photosynthetic pigments
Production of polyphenolic compounds in shoot cultures of Hypericum species characteristic for the Balkan flora
KALINA DANOVA
Pages 29-36

Full reference | Abstract | Full text PDF
    ABSTRACT:  Th is work compares the polyphenolic compounds content in the in vitro shoot cultures of Hypericum rumeliacum, H. tetrapterum and in the hypericin non-producing H. calycinum, which are characteristic for the Balkan fl ora. In addition, the impact of diff erent vitamin supplementation to culture medium on secondary metabolites is discussed. Th ough Gamborg’s vitamins supplementation improved growth parameters in vitro, it resulted in reduced polyphenolics levels in comparison with Murashige and Skoog’s vitamins. Preliminary experiments on cryopreservation of H. rumeliacum which were previously performed, gave low rates of plant survival. Th erefore, as a part of a broader study for improving the approach for cryopreservation of H. rumeliacum, phenolics accumulation, total soluble sugars content as well as malondialdehyde and hydrogen peroxide content were studied during preculture treatment prior to dehydratation and liquid nitrogen immersion and results are discussed in this work.

    KEY WORDS:  Hypericum rumeliacum, H. tetrapterum, H. calycinum, shoot cultures, polyphenolics, cryopreservation..